Core Labs

The Siteman Cancer Center Embryonic Stem Cell Core

PRIME CONTACT

Elaine Ross

LOCATION

Room #109 Biotech Bldg.

CAPABILITIES

Stable and transient electroporations into ES cells; ES cell selection, colony picking, freezing, expansions, and cryopreservation of homologous recombinant clones. WUMS internal resource for wild-type RW.4 and B6/BLU ES cells. Resource for Turbo-cre and pLOX-neo plasmids.

COST STRUCTURE

Standard transfection in RW.4 ES cells: $1000.00; in B6/BLU ES cells: $1500.00
Transient transfection: $1000.00
ES clone expansion: $50.00 per clone
Additional 24-well plates for analysis: $150.00 per plate
1 vial of RW.4 ES cells: $175.00 (p#10) limit of 6 vials per year
1 vial of B6/BLU ES cells: $250.00 (p#11) limit of 4 vials per year
1 vial of positive (LacZ) or negative (wild-type) control blood: $5.00 per vial

ADDITIONAL INFORMATION

Microinjections into Taconic B6 blastocysts: $3000.00 2 days each with 20 injected and implanted blastocysts
additional microinjection days: $1000.00 each

Washington University School of Medicine
Department of Medicine